HISTORY, RELEVANCE AND SCOPE OF MICROBIOLOGY AND CLASSIFICATION OF MICROORGANISMS

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Microorganisms


Microorganisms  are  organisms  too  small  to  be  seen  clearly  by  the unaided eyes. They are very small life forms so small that individual microorganisms  cannot  be  seen  without  magnification.  They  include fungi,  bacteria,  algae,  protozoa  and  viruses.  Some  microorganisms however,   like   the   eukaryotic   microorganisms   are   visible   without magnification. Examples are bread moulds and filamentous algae.

3. 2     Microorganisms are Cells


The cell is the fundamental unit of life; a single cell is an entity isolated from other cells. Two fundamental different types of cells exist among microorganisms; which are prokaryotic and eukaryotic.

.    Prokaryotes: These microbial cells lack membrane-bound nucleus
and organelles.
.    Eukaryotes: Possess a membrane-bound nucleus and organelles.

Table 1: Difference
Properties
es between prokaryotic and
Prokaryotic cell
eukaryotic cells                    
Eukaryotic cell

Nuclear
membrane
Absent
Present

Chromosome
Single, closed, circular, dsDNA
Multiple, linear, dsDNA

Ribosomes
70s (50s+30s)
80s (60s+40s)

Mitochondrion
Absent
Present

Examples
Bacteria
Fungi, plant cells, animal cells, algae









Figure 1: A prokaryotic (A) and eukaryotic cells (B).
Source: http://www.thepsimple-homeschool.com/imagefiles/labeled_cell.gif

3. 3    Classification Systems for Microorganisms


The  Five  Kingdom  System  of Classification  Based  on  cell  type  and mode  of nutrition,  there  was  an  establishment  of the  five  kingdoms system of classifying organisms in which we have:

i.
Monera

ii.
Protista

iii.
Fungi

iv.
Planta

v.
Animalia


 
Microorganisms except for viruses, which are acellular and have their own classification system, were placed in the first three kingdoms.
The   Three   Domains   System   of  Classification   Presently,   through advances in cell biology, biochemistry and genetics, microorganisms are now placed  into  three  domains,  each  of which  comprises  of various kingdoms.

The domains are:

i.         Bacteria (prokaryotic – “true bacteria”)
ii.         Archaea (prokaryotic – “ancient bacteria”)
iii.       Eucarya (eukaryotic)

Domain Bacteria

i.        They are prokaryotic.
ii.        They are single celled organisms.
iii.       They lack membrane bound nucleus and organelles.
iv.       Most have cell wall that contains peptidoglycan.
v.        They are  found  in the  soil, water  and  air  and  on  other  living
organisms.
vi.       Some are harmful while others are beneficial to man.

Domain Archaea

i.         They were formerly known as archaeobacteria.
ii.        They are prokaryotic.
iii.       They are single celled organisms.
iv.       They lack membrane bound nucleus and organelles.
v.        They lack peptidoglycan in their cell walls.
vi.       They have unique membrane lipids.
vii.      Some have unusual metabolic characteristics, e.g. methanogens
which  generate methane  gas. viii.  Many are  found  in  extreme environments.

The major differences between domain archaea and domain bacteria are:
i.         Differences in ribosomal RNA sequences.
ii.        The absence of cell wall peptidoglycan.
iii.       The presence of unique membrane lipids.

Domain Eucarya

The major groups  of microorganisms  in this domain are protists  and fungi.
Protists





These groups of microorganisms consist of unicellular algae, protozoa, slime moulds and water moulds.

Algae

i.         They are simple organisms.
ii.        Mostly unicellular.
iii.       They are photosynthetic together with cyanobacteria.
iv.       They produce about 75% of the plant’s oxygen.
v.        Commonly found in aquatic environment.
vi.       They are primary producers in food chains in aquatic habitat.

Protozoa

i.         They are unicellular.
ii.         Eukaryotic organisms and animal like.
iii.        They are usually motile.
iv.       Some are free living while some are pathogenic.

Slime Moulds

They are protists which have different forms at different stages of their life cycles. At a stage they are like protozoa and at another stage like fungi.

Water Moulds

These are found on the surface of fresh water and moist soils. They feed on decaying vegetation such as logs and mulch.

Fungi

i.         These are microorganisms that range from unicellular forms like
yeasts to moulds  and mushrooms which  are multicellular with thread like structures called hyphae.
ii.        They absorb nutrients from their environments.
iii.       Many play beneficial roles while others cause diseases in plants,
animals and human.

Viruses

i.         They are acellular entities (non-cellular).
ii.        They lack the fundamental structure of living cell but only carry
out functions of living organisms when in living cells.


iii.       They are the smallest of all the microorganisms (10,000 smaller
than a typical bacterium).
iv.       They can only be seen by the electron microscope.
v.        They cause many diseases of plants, animals and humans.
vi.       Entities are not placed in any of the domain but are classified on
a separate system.
vii.       They cause many diseases of plants, animals and humans.

SELF-ASSESSED EXERCISE


i.         Give three examples of diseases caused by each classification of
microorganism
ii.        Answer the following questions:
.    Define the term, microorganism (LO1)
.    Compare  and  contrast  prokaryotic  and  eukaryotic  cells
(LO3)
.    List the three  domains under which microorganisms are
classified (LO4)
.    List three characteristics each of the following domains:
.        Bacteria
.        Archaea
.        Fungi (LO5).
.    What  are the  differences between bacteria  and  archeae?
(LO5).
.    Why are viruses not placed in any of the domains? (LO6).

4.0     CONCLUSION


Microorganisms  are  life  forms  that  cannot be  seen with naked  eyes. They  include  bacteria,  viruses,  fungi  and  protozoans  placed  in  three main domains namely Bacteria, Archaea and Eucarya.

5.0     SUMMARY


In this unit, you have learnt about microorganisms, their classification systems and the three main domains where they are placed.

6.0     TUTOR-MARKED ASSIGNMENT


With the aid of a well labelled diagram, distinguish between a bacterial cell and a eukaryotic cell.



7.0     REFERENCES/FURTHER READING


Atlas, R.M. (1995). Microorganisms in Our World. Mosby Year Book. Inc.

Kayser,  F.H.,  Bienz,  K.A.,  Eckert,  J.  &  Zinkernagel,  R.N.  (2005). Kayser, Medical Microbiology. Thieme, Stuttgart, Germany

Markey, B., Leonard, F., Archambault, M., Cullinane, A. & Maguire, D. (2013). Clinical Veterinary Microbiology. 2nd  Edition. MOSBY ELSEVIER

Medigan, M.T. et al. (2009). Brock Biology of Microorganisms.  12th Edition. Pearson Education Inc. Pelczar, M.J., Chan, E.C.S. & Krieg, R.N. (2001). Microbiology. 5th Edition. McGraw-Hill.

Mitchell, R. & Gu, J.-D. (editors) (2010). Environmental Microbiology. 2nd   Edition.  Hoboken,  New  Jersey,  John  Wiley  &  Sons,  Inc, Wiley-Blackwell

Struthers,  K.  (2017).  Clinical  Microbiology.  2nd   Edition.  CRC  Press, Taylor & Francis Group.

Talaro,  K.P.  &  Talaro,  A.  (2002).  Foundations  in  Microbiology.  4th Edition. The McGraw-Hill Companies.

Willey, J.M., Sherwood, L.M & Woolverton, C.J. (2008). Microbiology. 7th   Edition.   Boston   Bur   Bridge,   IL:   McGraw-Hill   Higher Education.

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